Web4. A 0.4-nM solution of anti-His Tb-labeled antibody was prepared in 50 mM sodium HEPES 10 mM MgCl 2, 1 mM DTT, 0.05 mg/mL BSA, 0.015% (v/v) Brij-35 at pH 7.5 (Buffer F) and a separate solution of 2 nM TYK2-JH2 (double the final concentration) was also prepared in Buffer F. 5. 10 μL of the anti-His Tb-labeled antibody solution was added to the entire … WebWe thus investigated whether 3SM5 and 4N1H also competed with the ligand, as 238D2 does. The HTRF ratio showing the proximity between the receptor and its ligand was measured between a SNAP-tagged CXCR4 (coupled to the Tb donor) and fluorescent SDF1 before competitive experiments in the presence of the Nb (black dot, Figure 3A).

Terbium - an overview ScienceDirect Topics

Web8 mrt. 2024 · Here, the HTRF donor (Lumi4™-Tb cryptate) is used to label a monoclonal antibody specific for IP1. The HTRF acceptor-labeled IP1 competes with intracellular produced IP1 for the antibody binding ... WebHTRF® is based on Time-Resolved Fluorescence (TRF) and Fluorescence Resonance Energy Transfer (FRET). In HTRF® the lanthanide chelate is used as donor and is either Europium cryptate (Eu 3+ cryptate) or Lumi4™-Tb (Tb 2+ cryptate), and the acceptor is either XL665, a modified allophycocyanin, or d2, a second generation of acceptor. movie the other man with liam neeson

JP7228586B2 - Aminoimidazopyridazines as kinase inhibitors

WebCellul'erk 为基于 HTRF 技术的检测试剂方法，可用于磷酸化 AKT （ Ser473 ）含量的检测，此试剂可直接在完整细胞中检测活化的 ERK1/2 和 AKT 。 当受体被刺激后，引发了相关激酶的活化，通过该试剂盒就可检测到细胞裂解液中磷酸化蛋白的含量。 Web19 uur geleden · Dysk serwerowy Supermicro DYSK HDD SATA 400GB 3.5 - (ST3400620NS) - od 194,34 zł, porównanie cen w 1 sklepach. Zobacz inne Dyski serwerowe, najtańsze i najlepsze oferty, opinie. WebHTRF® readout can be achieved by PHERAstar FSX after the installation of the HTRF® dedicated optical block which includes the optimized excitation and emission filters, the … movie the others explained