Cisplatin apoptosis induction concentration

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August undefined, 2024

WebJan 16, 2024 · In the experimental group, with a concentration of 0.1 g/ml of cisplatin, about 37% of the cells were alive and about 6% of the cells had primary apoptosis. … WebOct 5, 2014 · Cisplatin is stable under normal temperatures and pressures, but may transform slowly over time to the trans-isomer (IARC 1981, Akron 2009). Cisplatin has a …

Cisplatin at concentrations of 1 μM and 2 μM induces

WebMay 3, 2024 · MTC induces apoptosis in ovarian cancer cells. (a) Photomicrographs of ES-2 cells treated with MTC for 24 h showed the concentration-dependent effect on cytotoxicity. Immunofluorescent staining to identify cleaved-PARP was performed to represent caspase activity. WebMay 16, 2011 · After these metabolic changes a drastic decrease in cellular impedance occurred at about 10–11 h after exposure to cisplatin ( Fig. 2 C ), which reflects the … flower shop bromsgrove high street

Necrotic Concentrations of Cisplatin Activate the …

WebFeb 17, 2024 · It came out that when the concentration of cisplatin was below 2 mg/L, the cell activity was basically above 90%, so the effect of cisplatin on cell activity was small at this time. However, when the concentration of cisplatin was above 2 mg/L, the cell activity was inversely proportional to the concentration of cisplatin. WebCell clones were stained by Giemsa; (c) Induction of apoptosis and necrosis was analysed in untreated LTTs and those treated with IC 50 concentration of cisplatin, IC 50 … WebApr 28, 2011 · Cisplatin is a chemotherapeutic drug widely used in the treatment of a variety of cancers, including sarcomas, lymphomas, and some carcinomas such as small … green bay downtown history

Cellular potassium ion deprivation enhances apoptosis induced by cisplatin.

Acute apoptosis by cisplatin requires induction of reactive oxyge…

WebMar 1, 2006 · The chloride ions in cisplatin are particularly important in its action as a drug. They are relatively stable when the drug is outside the cell, where the chloride concentration is normally high. But when the drug gets inside the cell and the chloride concentration drops, cisplatin loses its two chlorides, and they are replaced by water … WebOct 13, 2024 · Cisplatin is mainly cleared by the kidney through glomerular filtration and tubular excretion, resulting in a higher concentration of this drug in the kidney than in … flower shop brewster nyWebCisplatin activates multiple signal transduction pathways involved in coordinating cellular responses to stress. Here we demonstrate a requirement for extracellular signal-regulated protein kinase (ERK), a … green bay downtown library

"WebMar 17, 2016 · Induction of ROS-dependent apoptosis by cisplatin combined with gallic acid (GA) in H446 cells. (A) Apoptosis assay by flow cytometry using PI and Annexin V-FITC double staining. H446 cells were treated with or without 6 mM NAC for 2 h before challenge with 5 µg/ml cisplatin combined with 3 µg/ml GA for 24 h. " - Cisplatin apoptosis induction concentration

Cisplatin apoptosis induction concentration

Current Strategies to Combat Cisplatin-Induced Ototoxicity

WebJul 5, 2024 · The obtained results from flow cytometry analysis showed that the rate of apoptosis was 4.26% in the cells treated with cisplatin, 38.69% in the cells treated with cisplatin-IONPs and 46.33% in the cells treated with cisplatin-IONPs-chitosan after 24 h (Fig. 3) and also showed that the rate of necrotic cells treated with cisplatin was … WebSep 30, 2024 · Induction of apoptosis and DNA damage response were evaluated in both monotherapy and combination therapy groups. Results: Results demonstrate that …

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WebJul 3, 2024 · A single injected dose of cisplatin has been commonly used in that it is effective and practical at concentrations ranging from 10 to 30 mg/kg, as shown in Table 1. However, this approach is not similar to clinical chemotherapy in which cisplatin is administered as a single intravenous dose every 4 to 6 weeks with as many as six … WebCisplatin is the most important and efficacious chemotherapeutic agent for the treatment of advanced gastric cancer. Cisplatin forms inter- and intrastrand crosslinked DNA adducts …

WebAug 16, 2002 · Induction of apoptosis with MMC in the human breast cancer cell line MCF-7. To determine the cytotoxic effect of various anticancer drugs with different mechanisms of action in MCF-7 cells, the ... WebApr 14, 2024 · Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different …

WebCisplatin-induced testicular damage is a major obstacle in the application of cisplatin as chemotherapeutic agent. However, it remains as one of the most widel 掌桥科研一站式科研服务平台

WebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung carcinoma (NSCLC). However, some patients fail to respond to the DDP chemotherapy. ... adenocarcinoma promotes proliferation via the accumulation of β-catenin 17 and regulates lung tumorigenesis through cell apoptosis and autophagy pathways. 16 USP14 is critical ...

WebA high concentration of cisplatin induces apoptosis in many tumor cell lines. Whether cisplatin induces apoptosis even in a controlled release formulation has not been … flower shop bristol flowers delivery bristolWebOct 16, 2006 · Cisplatin is a broad-spectrum anticancer drug that is also widely used in experimental studies on DNA damage-induced apoptosis. Induction of apoptosis … flower shop brisbaneWebApr 28, 2011 · For all cell lines, it is true that concentrations of cisplatin up to 30μM caused mainly a mode of cell death with clear morphological and biochemical characteristics of apoptosis, whereas the commonest phenotype observed with concentrations equal or higher than 300μM had clearly lost many of these characteristics and more closely … green bay downtown mapWebApr 25, 2014 · The dosage of cisplatin determines whether the cells die by necrosis or apoptosis [30]. In cell culture studies, high concentrations of cisplatin cause necrotic cell death but lower concentrations lead to apoptosis. However,in vivo, cisplatin induces both necrosis and apoptosis [31]. green bay downtown barsWebWe previously described that blocking ALDH activity enhances the induction of apoptosis, especially in ovarian cancer stem cells treated with chemotherapeutic agents. ... green bay dmv phone numberWebSep 30, 2024 · Both cisplatin and onalespib were diluted further in complete media for assay dependent concentrations. The final DMSO concentration was 0.005% (v/v) for 3000 nM Onalespib, 0.0002% (v/v) and 0.00008% (v/v) for 100 nM for 50 nM, respectively. XTT Cell Viability Assays green bay downtown parkingWebFor apoptosis detection, flow cytometry was used to verify whether TAS induced apoptosis in NSCLC cells. The apoptosis rate was significantly higher in response to an increasing dose of TAS in A549 and H1299 cells (Fig. 2 D-2E). Taken together, these findings suggested that TAS has good anticancer activity. green bay downtown restaurants