Biotinylated mouse tim4-fc

WebMar 12, 2014 · TIM4 is the main receptor mediating the phagocytosis of PtdSer-bearing targets in murine macrophages. Bone marrow-derived macrophages (BMDMs) from TIM4 − / − mice were reported to have a reduced ability to engulf apoptotic bodies (Martinez et al., 2011).This observation suggests that TIM4 may be the primary PtdSer receptor in these … WebAug 31, 2024 · The EVs were isolated from the sera of PC patients or NC in cohort-1 using the Magcapture Exosome Isolation Kit (Fujifilm) according to the manufacturer’s protocol. In brief, 0.6 mg of streptavidin magnetic beads, bound with 1 µg of biotinylated mouse Tim4-Fc, was added to the serum supplement and rotated overnight at 4 °C.

TIM4-Affinity Methods Targeting Phosphatidylserine for

WebWe offer Anti-Mouse secondary antibodies in a range of host species including Alpaca, Donkey, Goat, Rabbit, Rat and Sheep. JIR secondary antibodies are available as whole IgG and F (ab') 2 fragment formats. Fab fragment formats are also available and can be an elegant component of multiple blocking and labeling protocols. WebMeaning of biotinylated. What does biotinylated mean? Information and translations of biotinylated in the most comprehensive dictionary definitions resource on the web. dfe teacher services employers https://danielanoir.com

Tim-4 (human): FC (human) - Sigma-Aldrich

WebMouse Ig cocktail. Formulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation. The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. Concentration. 0.5 mg/ml. Storage & Handling. The antibody solution should be stored undiluted between 2°C and 8°C. WebTo produce an mAb against Tim4, Armenian hamsters were immunized with Tim4-Fc, in which the extracellular region of mouse Tim4 was joined to human IgG Fc region (11). Lymphocytes from the immunized hamsters were fused with a mouse myeloma NSObcl2 (17). One hybridoma (Mat4-9-5) that was suitable for immunohistochemical staining, WebJun 29, 2024 · An ELISA plate (AGC Techno Glass, Shizuoka, Japan) was coated with 1 μg/mL recombinant mouse TIM4-Fc protein (FUJIFILM Wako Pure Chemical)/50 mM carbonate-bicarbonate buffer (pH 9.6) and ... churchwood surgery

Mouse IgG1 Biotinylated Antibody IC002B: R&D Systems

Category:Mouse TIM4/Human Fc Chimera, recombinant

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Biotinylated mouse tim4-fc

Mouse macrophages show different requirements for …

WebMay 19, 2024 · 2.3 TIM4-Affinity Beads. 1. Mouse TIM4-Fc protein. 2. Maleimide-PEG11-Biotin: 25 mM Maleimide-PEG11-Biotin in dimethylsulfoxide (DMSO). To prepare 250 … WebMonoclonal Hamster‑Armenian anti‑Mouse TIMD4 / TIM4 / TIM‑4 Antibody (FITC, IF) LS‑C663029. ... FC: Cells were incubated on ice for 30 min with 1 ug/ml biotinylated Kat5-18 in 100 ul staining solution (PBS containing 2% FCS), followed by incubation with 2 ug/ml Allophycocyanin (APC)-Cyanine 7 (Cy7)-conjugated streptavidin and 2 ug/ml ...

Biotinylated mouse tim4-fc

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WebRegarding Tim4 expression, cells were stained with PE-labeled cIg, or RMT4-54 (anti-Tim4: BioLegend). Mouse peritoneal resident cells were pretreated with clone 93 (anti-CD16/32: BioLegend) to block Fc receptors, and then stained with FITC-anti-CD11b (BioLegend) and the above mAbs. These cells were analyzed by an Accuri C6 cytometer (BD ... WebTIM-4 (T cell; immunoglobulin; Mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIM-4 is exclusively expressed on antigen-presenting cells, where it mediates phagocytosis of apoptotic cells and plays an important role in maintaining tolerance. TIM-4 binds specifically to TIM-1 which is also ...

WebSpecificity / Sensitivity. Anti-mouse IgG (H+L), (Biotinylated) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins … WebNov 10, 2024 · Mouse FcRn / FCGRT&B2M Heterodimer Protein, His Tag (MALS & BLI verified) FCM-M82W5: HEK293: Biotinylated Mouse FcRn / FCGRT&B2M Heterodimer Protein, His, Avitag™ (MALS & BLI verified) FCM-M82W6 ...

WebFeb 9, 2024 · Tim4 is involved in recognition of MWCNTs by mouse peritoneal macrophages. (A) Recognition of MWCNTs (30 μg/mL) by WT or Tim4−/− mouse … WebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT-PCR with mRNA from mouse liver and …

WebOct 24, 2007 · Mouse Tim4 is a type I membrane protein, consisting of a signal sequence and extracellular, ... Tim4-Fc-coated latex beads incubated with Tim1-Fc in the absence …

WebThe cells were then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b (Cat. No. 550993/561114) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse TIM-4 antibody (Right Panel). Two-color flow cytometric contour plots showing the correlated expression patterns of TIM-4 (or Ig Isotype control staining ... dfe teachers pay and conditions 2022WebSep 23, 2016 · Nevertheless, Tim4-Fc-conjugated beads were still able to isolate sEVs efficiently from 10K sup of pMac which abundantly express … church woodstockWebBackground: IgG1. R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. dfe teachers pay scales 2022 2023WebFUJIFILM biotinylated mouse tim4 fc Biotinylated Mouse Tim4 Fc, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … church woodsWebApr 1, 2024 · Exosome concentration by ultracentrifugation and Tim4 affinity columns We also concentrated Exo by the Tim4-affinity method using a MagCapture Exosome Isolation Kit PS (Wako, Japan). Streptavidin magnetic beads (0.6 mg), bound with 1 μg of biotinylated mouse Tim4-Fc, were added to precleared CSs supplemented with 2 mM … churchwood surgery pontypool emailWebMouse Tim4–Fc was prepared as described . To produce mProS and mGas6, full-length cDNAs for mProS (Gene ID: 19128) and mGas6 (Gene ID: 14456) were prepared by RT … dfe teachingWebTim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 … dfe teachers pension grant